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b1d8  (Novus Biologicals)


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    Novus Biologicals b1d8
    B1d8, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b1d8/product/Novus Biologicals
    Average 90 stars, based on 1 article reviews
    b1d8 - by Bioz Stars, 2026-03
    90/100 stars

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    Recovery rates of spiking experiments using (A) MCF‐7, (B) MDA‐MB‐231, and (C) SHP‐77 cells. Of each cell line, either 1, 10, or 100 cells were spiked into 7.5 mL of HD blood. Experiments were performed in triplicate. Twenty‐five Hs 578T (D), MDA‐MB‐231 (E), and MDA‐MB‐468 (F) cells were spiked into 7.5 mL of HD blood to analyze the impact of membrane functionalization with an anti‐EGFR antibody on cell lines with intermediate to high EGFR and low to high EpCAM expression. All error bars represent SD.

    Journal: International Journal of Cancer

    Article Title: Fast enrichment and detection of circulating tumor cells from large volumes of whole blood of breast cancer patients utilizing a functionalized bioaffinity CTC filtration membrane

    doi: 10.1002/ijc.70216

    Figure Lengend Snippet: Recovery rates of spiking experiments using (A) MCF‐7, (B) MDA‐MB‐231, and (C) SHP‐77 cells. Of each cell line, either 1, 10, or 100 cells were spiked into 7.5 mL of HD blood. Experiments were performed in triplicate. Twenty‐five Hs 578T (D), MDA‐MB‐231 (E), and MDA‐MB‐468 (F) cells were spiked into 7.5 mL of HD blood to analyze the impact of membrane functionalization with an anti‐EGFR antibody on cell lines with intermediate to high EGFR and low to high EpCAM expression. All error bars represent SD.

    Article Snippet: After an initial washing step with 20 mL of 0.1% Tween/PBS, the filter, which had been functionalized with streptavidin, was incubated with 1 μg/mL biotinylated anti‐EpCAM‐antibody (R&D Systems) or 1 μg/mL biotinylated anti‐EGFR‐antibody (clone B1D8, Biotium) at RT for 1 h. Subsequently, the filter was washed with 20 mL of 0.1% Tween/PBS, and residual Tween was removed by washing the complete filtering system with 100 mL PBS.

    Techniques: Membrane, Expressing